Figure 2: Evaluation of the phosphorylation profile of sMyBP C in young and old wild type and mdx FDB muscles.(a) Standard SDS PAGE western blot analysis of protein lysates prepared from young (2 months) and old (14 months) wild type and mdx FDB muscles. Lysates were probed with a pan sMyBP C (top panel) recognizing all sMyBP C variants, and Hsp90 (bottom panel) to ensure equal loading. (a’) Percent expression of total sMyBP C in young and old wild type and mdx FDB muscles, after normalization to the levels of Hsp90, and relative to the percent expression of sMyBP C in young wild type tissue, which was set as baseline.
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