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(E) Upper: Schematic description of the domains of hSSB1. Lower: HEK293T cells co transfected HA p53 with various hSSB1 mutants as indicated for 24 h were lysed with MCLB, and were analyzed as A.Full figure and legend (75K)Next, we attempted to determine the regions responsible for the interaction between hSSB1 and p53. A series of deletions fake ray bans of hSSB1 and p53 were generated (Figure 2D and 2E), and co IP was performed to map the binding domains of these two proteins.
Analysis of meiotic progenyThe segregation of mutant phenotypes in meiotic crosses allows one to determine whether a phenotype is due to a mutation in a single gene. Crosses involving multiple mutations can be used to map the mutations with respect to each other and also to construct strains with different combinations of mutations to examine the functional relationships between genes. A.
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